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h2b hibit  (Twist Bioscience)

 
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    Twist Bioscience h2b hibit
    Activation-dependent Gα q translocation into the nucleus. A , f-Gα q localizes strongly to the PM while ( B ) SMARCD3-V5 localizes strongly to the nucleus in HEK293 cells. C , constitutive activation of Gα q enhances nuclear residency and is prevented by tethering Gα q to the PM. HEK293 cells stably expressing <t>Histone</t> <t>H2B-HiBiT</t> were transfected with the indicated Gα q -LgBiT cDNA containing plasmids. In parallel cells were co-transfected with the indicated Gα q -LgBiT cDNAs and GFP-HiBiT to account for differences in expression. Twenty four hour later cells were assessed for luminescence complementation. Data are corrected for differences in expression based on GFP HiBiT. N = 7. Data were analyzed using a one-way ANOVA with Sidak’s multiple comparisons post-test (relevant p -values shown). Raw luminescence values: GFP-HiBiT: 20 × 10 6 ± 5 × 10 6 (SEM), N = 3 vs. H2B HiBiT: 0.22 ×10 6 ± 0.08 × 10 6 , N = 7, indicate 1% of the Gα q is present in the nucleus. D , activation of M3 muscarinic receptors enhances Gα q -LgBiT nuclear accessibility. HEK293 cells stably expressing Histone H2B-HiBiT were transfected with Gα q (wt) -LgBiT and M3 muscarinic receptor cDNA plasmids. Twenty four hour later cells were stimulated for 3 h with the indicated concentrations of carbachol followed by measurement of luminescence complementation.
    H2b Hibit, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h2b+hibit/pmc13129537-190-0-4?v=Twist+Bioscience
    Average 86 stars, based on 1 article reviews
    h2b hibit - by Bioz Stars, 2026-08
    86/100 stars

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    1) Product Images from "G protein Gα q subunits engage targets in the nucleus involved in chromatin remodeling and gene expression"

    Article Title: G protein Gα q subunits engage targets in the nucleus involved in chromatin remodeling and gene expression

    Journal: The Journal of Biological Chemistry

    doi: 10.1016/j.jbc.2026.111322

    Activation-dependent Gα q translocation into the nucleus. A , f-Gα q localizes strongly to the PM while ( B ) SMARCD3-V5 localizes strongly to the nucleus in HEK293 cells. C , constitutive activation of Gα q enhances nuclear residency and is prevented by tethering Gα q to the PM. HEK293 cells stably expressing Histone H2B-HiBiT were transfected with the indicated Gα q -LgBiT cDNA containing plasmids. In parallel cells were co-transfected with the indicated Gα q -LgBiT cDNAs and GFP-HiBiT to account for differences in expression. Twenty four hour later cells were assessed for luminescence complementation. Data are corrected for differences in expression based on GFP HiBiT. N = 7. Data were analyzed using a one-way ANOVA with Sidak’s multiple comparisons post-test (relevant p -values shown). Raw luminescence values: GFP-HiBiT: 20 × 10 6 ± 5 × 10 6 (SEM), N = 3 vs. H2B HiBiT: 0.22 ×10 6 ± 0.08 × 10 6 , N = 7, indicate 1% of the Gα q is present in the nucleus. D , activation of M3 muscarinic receptors enhances Gα q -LgBiT nuclear accessibility. HEK293 cells stably expressing Histone H2B-HiBiT were transfected with Gα q (wt) -LgBiT and M3 muscarinic receptor cDNA plasmids. Twenty four hour later cells were stimulated for 3 h with the indicated concentrations of carbachol followed by measurement of luminescence complementation.
    Figure Legend Snippet: Activation-dependent Gα q translocation into the nucleus. A , f-Gα q localizes strongly to the PM while ( B ) SMARCD3-V5 localizes strongly to the nucleus in HEK293 cells. C , constitutive activation of Gα q enhances nuclear residency and is prevented by tethering Gα q to the PM. HEK293 cells stably expressing Histone H2B-HiBiT were transfected with the indicated Gα q -LgBiT cDNA containing plasmids. In parallel cells were co-transfected with the indicated Gα q -LgBiT cDNAs and GFP-HiBiT to account for differences in expression. Twenty four hour later cells were assessed for luminescence complementation. Data are corrected for differences in expression based on GFP HiBiT. N = 7. Data were analyzed using a one-way ANOVA with Sidak’s multiple comparisons post-test (relevant p -values shown). Raw luminescence values: GFP-HiBiT: 20 × 10 6 ± 5 × 10 6 (SEM), N = 3 vs. H2B HiBiT: 0.22 ×10 6 ± 0.08 × 10 6 , N = 7, indicate 1% of the Gα q is present in the nucleus. D , activation of M3 muscarinic receptors enhances Gα q -LgBiT nuclear accessibility. HEK293 cells stably expressing Histone H2B-HiBiT were transfected with Gα q (wt) -LgBiT and M3 muscarinic receptor cDNA plasmids. Twenty four hour later cells were stimulated for 3 h with the indicated concentrations of carbachol followed by measurement of luminescence complementation.

    Techniques Used: Activation Assay, Translocation Assay, Stable Transfection, Expressing, Transfection



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    h2b hibit  (Twist Bioscience)
    86
    Twist Bioscience h2b hibit
    Activation-dependent Gα q translocation into the nucleus. A , f-Gα q localizes strongly to the PM while ( B ) SMARCD3-V5 localizes strongly to the nucleus in HEK293 cells. C , constitutive activation of Gα q enhances nuclear residency and is prevented by tethering Gα q to the PM. HEK293 cells stably expressing <t>Histone</t> <t>H2B-HiBiT</t> were transfected with the indicated Gα q -LgBiT cDNA containing plasmids. In parallel cells were co-transfected with the indicated Gα q -LgBiT cDNAs and GFP-HiBiT to account for differences in expression. Twenty four hour later cells were assessed for luminescence complementation. Data are corrected for differences in expression based on GFP HiBiT. N = 7. Data were analyzed using a one-way ANOVA with Sidak’s multiple comparisons post-test (relevant p -values shown). Raw luminescence values: GFP-HiBiT: 20 × 10 6 ± 5 × 10 6 (SEM), N = 3 vs. H2B HiBiT: 0.22 ×10 6 ± 0.08 × 10 6 , N = 7, indicate 1% of the Gα q is present in the nucleus. D , activation of M3 muscarinic receptors enhances Gα q -LgBiT nuclear accessibility. HEK293 cells stably expressing Histone H2B-HiBiT were transfected with Gα q (wt) -LgBiT and M3 muscarinic receptor cDNA plasmids. Twenty four hour later cells were stimulated for 3 h with the indicated concentrations of carbachol followed by measurement of luminescence complementation.
    H2b Hibit, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/h2b+hibit/pmc13129537-190-0-4?v=Twist+Bioscience
    Average 86 stars, based on 1 article reviews
    h2b hibit - by Bioz Stars, 2026-08
    86/100 stars
    		  Buy from Supplier

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    Activation-dependent Gα q translocation into the nucleus. A , f-Gα q localizes strongly to the PM while ( B ) SMARCD3-V5 localizes strongly to the nucleus in HEK293 cells. C , constitutive activation of Gα q enhances nuclear residency and is prevented by tethering Gα q to the PM. HEK293 cells stably expressing Histone H2B-HiBiT were transfected with the indicated Gα q -LgBiT cDNA containing plasmids. In parallel cells were co-transfected with the indicated Gα q -LgBiT cDNAs and GFP-HiBiT to account for differences in expression. Twenty four hour later cells were assessed for luminescence complementation. Data are corrected for differences in expression based on GFP HiBiT. N = 7. Data were analyzed using a one-way ANOVA with Sidak’s multiple comparisons post-test (relevant p -values shown). Raw luminescence values: GFP-HiBiT: 20 × 10 6 ± 5 × 10 6 (SEM), N = 3 vs. H2B HiBiT: 0.22 ×10 6 ± 0.08 × 10 6 , N = 7, indicate 1% of the Gα q is present in the nucleus. D , activation of M3 muscarinic receptors enhances Gα q -LgBiT nuclear accessibility. HEK293 cells stably expressing Histone H2B-HiBiT were transfected with Gα q (wt) -LgBiT and M3 muscarinic receptor cDNA plasmids. Twenty four hour later cells were stimulated for 3 h with the indicated concentrations of carbachol followed by measurement of luminescence complementation.

    Journal: The Journal of Biological Chemistry

    Article Title: G protein Gα q subunits engage targets in the nucleus involved in chromatin remodeling and gene expression

    doi: 10.1016/j.jbc.2026.111322

    Figure Lengend Snippet: Activation-dependent Gα q translocation into the nucleus. A , f-Gα q localizes strongly to the PM while ( B ) SMARCD3-V5 localizes strongly to the nucleus in HEK293 cells. C , constitutive activation of Gα q enhances nuclear residency and is prevented by tethering Gα q to the PM. HEK293 cells stably expressing Histone H2B-HiBiT were transfected with the indicated Gα q -LgBiT cDNA containing plasmids. In parallel cells were co-transfected with the indicated Gα q -LgBiT cDNAs and GFP-HiBiT to account for differences in expression. Twenty four hour later cells were assessed for luminescence complementation. Data are corrected for differences in expression based on GFP HiBiT. N = 7. Data were analyzed using a one-way ANOVA with Sidak’s multiple comparisons post-test (relevant p -values shown). Raw luminescence values: GFP-HiBiT: 20 × 10 6 ± 5 × 10 6 (SEM), N = 3 vs. H2B HiBiT: 0.22 ×10 6 ± 0.08 × 10 6 , N = 7, indicate 1% of the Gα q is present in the nucleus. D , activation of M3 muscarinic receptors enhances Gα q -LgBiT nuclear accessibility. HEK293 cells stably expressing Histone H2B-HiBiT were transfected with Gα q (wt) -LgBiT and M3 muscarinic receptor cDNA plasmids. Twenty four hour later cells were stimulated for 3 h with the indicated concentrations of carbachol followed by measurement of luminescence complementation.

    Article Snippet: H2B-HiBiT was synthesized by Twist Biosciences and was designed with the HiBiT sequence (VSGWRLFKKIS) followed by the V5 Tag, followed by H2B and inserted into pTwistCMV.

    Techniques: Activation Assay, Translocation Assay, Stable Transfection, Expressing, Transfection